Quantitative analysis of viral loads in canine herpesvirus, parainfluenza canin, canine respiratory adenovirus type 2 and bacterial load in Bordetella bronchiseptica and Mycoplasma cynos (pathogens involved in kennel cough complex or CIRD), responsible for respiratory problems, of varying intensity, in puppies or young adults.
Test indications
- Etiological diagnosis in a context of respiratory disorders of varying intensity in an isolated dog or within a group of puppies or possibly adult dogs.
- Assessment of epidemiological risk (identification of viral and/or bacterial circulation in the community) and environmental contamination.
Test characteristics
- Real-time RT-PCR
- Detection threshold
- Quantitative analysis of bacterial or viral loads
Samples to be taken
- Respiratory signs: deep nasal and oropharyngeal sampling (with a cytobrush or with a dry swab (on a dry tube) (+ whole blood on an EDTA tube).
- Deep respiratory signs: tracheobronchial or bronchoalveolar washing fluid on EDTA tube or dry tube (+ whole blood on EDTA tube).
- Pleural effusion
- Postmortem: tracheal or respiratory tract sample, lung on a dry tube.
- Environmental swab(s), in dry tube.
- Collect if possible before initiating antibiotic treatment.
Interpretation of the result
- Negative result : absence of virus and/or bacteria or quantity below the detection threshold. The agents sought are not involved in the observed pathology.
- Positive result
- Low or very low viral and/or bacterial load : chronic evolution or viral and/or bacterial carriage without clinical significance or sample taken during or after antiviral or antibiotic treatment. The load must also be interpreted according to the nature of the sample taken (pathogens associated with cells).
- Medium, high or very high viral and/or bacterial load : these charges are likely to explain the clinical signs observed.
- The prevalence of these pathogens being more or less high in the community, it is important to take into account epidemiological and clinical elements to interpret the results of viral and/or bacterial loads.
- Recent or ongoing antibiotic treatment at the time of collection may modify the sensitivity of the test and/or the bacterial load.
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