Quantitative detection (real-time PCR) of parvovirus allowing a definitive diagnosis of canine parvovirus, including in an animal recently vaccinated, particularly with vaccines with a high viral titer.
Test indications
- Diagnostic canine parvovirus.
- Evaluation you epidemiological risk (highlighting viral circulation in livestock).
- Evaluation of the environmental contamination and followed by decontamination in an infected environment.
Test characteristics
- Real-time RT-PCR
- Detection threshold
- Analyse quantitative.
- Strain typing possible on request (this typing is not routinely necessary for diagnosis).
Samples to be taken
- Ecouvillon rectal (even in post mortem, dry swab without transport medium).
- Environmental swab.
Interpretation of the result
Most dogs carry parvovirus in greater or lesser quantities in the community.. The presence of viruses, whether wild or of vaccine origin does not allow a definitive diagnosis to be made in the absence of quantification of the viral load excreted. Quantitative results must be interpreted in a clinical and epidemiological context.
- Negative result: Absence of virus or quantity below the detection threshold of the method.
- Positive result :
- Low or very low viral load : virus carriage or vaccination trace. This result makes it possible to exclude parvovirus.
- Average viral load : éventuellement compatible avec une vaccination récente avec un vaccin à haut titre viral. Le plus souvent les charges moyennes sont rencontrées dans les cas de co-infection parvovirus/coronavirus ou lors de formes subaiguës de parvovirose (évolution > 10 jours).
- High or very high viral load: compatible with parvovirus. Such loads can also be found in rare cases in asymptomatic animals in highly contaminated communities (presence of clinical cases).
Do not hesitate to Contact Us for an interpretation of the quantitative result