Quantitative analysis of viral load in canine herpesvirus responsible for mortality in the neonatal period (5 days to 3 weeks) and respiratory problems especially in young animals (kennel cough syndrome) as well as fertility problems in breeding animals. This virus is also involved in the appearance and progression of certain conjunctivitis and keratitis lesions.
Test indications
- Diagnosis of herpesvirus in its different clinical forms.
- Evaluation you epidemiological risk (demonstration of viral circulation in the community and detection of carriers).
Test characteristics
- Real-time RT-PCR
- Detection threshold
- Quantitative load analysis.
Samples to be taken
- Oropharyngeal, conjunctival and nasal cells (swabs or cytobrushes) in the presence of respiratory signs (possibility of looking for other agents of kennel cough syndrome, also attach blood on EDTA and/or broncho-alveolar washing liquid on a dry tube).
- Oropharyngeal cells and vaginal/prepucial cells (swabs or cytobrushes) or sperm (dry tube) in the presence of reproductive disorders in adults, to detect a port.
- Conjunctival and corneal cells (swabs or cytobrushes) in the presence of ocular lesions.
- Kidney, spleen, liver, lung (not fixed) post-mortem in case of neonatal mortality.
Interpretation of the result
- Negative result: Absence of herpesvirus in the sample or quantity below the detection threshold.
- Positive result : Compatible with herpesvirus disease (if high viral load) or carriage/re-excretion after reactivation of the latent virus (if low viral load).
Recent or ongoing antibiotic treatment at the time of sampling may modify the sensitivity of the test and/or the viral load.
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