Search for leishmanies of the complex Leishmania donovani (L. infantum, L. donovani, L. chagasi).
Test indications
- Diagnostic leishmaniasis in all its forms, including chronic.
- Status Determination of an asymptomatic animal.
- Treatment monitoring.
Test characteristics
- Real-time RT-PCR
- Detection threshold
- Quantitative analysis on request (according to the samples analyzed).
Samples to be taken
- Clinical suspicion – according to the clinical signs observed:
sang EDTA and or skin scraping and or lymph node puncture (nodal hypertrophy) and/or conjunctival cells. It is recommended to carry out several samples to carry out a single analysis on the mixture. - Determination of status of an asymptomatic animal : preferably bone marrow on EDTA.
- Treatment monitoring: EDTA blood (the blood turns negative first) then bone marrow if blood negative.
Interpretation of the result
- Negative result: Absence of leishmania or quantity below the detection threshold, allows leishmaniasis to be excluded if adequate samples have been taken.
- Positive result : Presence of leishmania, compatible with leishmaniasis or carriage in an endemic area. In dogs, the rate of asymptomatic carriers in the blood assessed with the Scanelis test is 6%. If a skin sample has been taken, the interpretation of the result must take into account the quantity of leishmania detected (contact us for an interpretation of your result).
- Special case of treatment monitoring : monitoring can be carried out on the blood which is the sample which is negative first. After an initial negative blood result, bone marrow is the most suitable sample for follow-up. The animal can be considered negative after two negative bone marrow tests 3 months apart.
Do not hesitate to Contact Us for an interpretation of the quantitative result
