La PCR - Scanelis

The PCR

The Forms (Polymerase Chain Reaction) is a method of direct detection of the genome of infectious agents, or parasites, by enzymatic amplification of a part of it. This molecular biology tool is very specific and makes it possible to reproducibly detect very small quantities of pathogens in samples of various kinds.

In genetics, PCR is a first step in the study of a given genomic region. It makes it possible to target this region to look for polymorphisms associated with a disease or a particular susceptibility to certain infectious agents or drugs.

Scanelis uses the Real-time PCR which has major advantages over conventional PCR methods:

quantitative analysis results if needed

strain typing using specific fluorescent probes
(e.g. vaccine strains/field strains)

reproducibility

sensitivity very important

Outcome Security (no risk of contamination and of false positives due to lack of electrophoresis analysis)

Automation of analysis

The development of real-time PCR tests requires real Biomolecular Expertise. These very specific tests must be adapted to the agents who circulate in the field. At the time of development, but also during the use of the test as a routine, Scanelis ensures the adequacy of the Specifications of its tests with the needs of the users.

Animation “ Real-time PCR»"
Presentation and comparison of PCR/PCR techniques in real time in 2 minutes

For more information on the PCR technique, its interest and applications in the veterinary field, you can also consult our publications and this article.

quantitative PCR

Discover the principle of PCR and quantitative PCR (real-time PCR) through this animation. Why choose quantitative PCR as a direct diagnostic technique?

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