Sanitary control
All tests below are real-time PCR tests, developed and validated by Scanelis.
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Helicobacter spp. and species typing Helicobacter hepaticus Parvovirus rodents sp. z z z + Virus identification: RMV, RPV, KRV and H1-Toolan (rat) / MVM and MPV (mouse) Murine Norovirus Bordetella bronchiseptica Chlamydia psittaci |
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VHD |
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Distemper Toxoplasma gondii Neospora caninum Bornavirus Leishmania Ehrlichia / Anaplasma Borrelia Hemobartonella Rubarth's hepatitis (CAV-1) Canine herpes virus (CHV) Canine Parvovirus (CPV-2) Canine Coronavirus Minute Virus (CPV-1) Bordetella bronchiseptica Respiratory adenovirus. (CAV-2) Canine Parainfluenza (CAP) Oral papillomavirus Leptospira interrogans Brucella spp. |
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FeLV IVF Toxoplasma gondii Hemobartonella Feline Coronavirus / FIP Feline parvovirus (typhus, panleukopenia) Bornavirus Herpes virus (FHV-1) Calicivirus (FCV) Chlamydophila psittaci / felis Bordetella bronchiseptica Leishmania Poxvirus |
Biological product quality control
Most of the tests available in the Scanelis catalogue can be used to control the quality of your organic products.
The real-time PCR tests available from Scanelis have been developed according to the recommendations of the European Pharmacopoeia concerning PCR tests (Methods of analysis 2.6.21 Nucleic Acid Amplification Techniques) and according to the standard NF-U47-600-2 (Methods of analysis in animal health - PCR - Requirements and recommendations for the development and validation of PCR in animal health).
We also offer:
- to validate the use of a test from our catalog in relation to your reference method,
- to develop specific tests for a particular need (e.g. detection of a vaccine strain).
Feel free to Contact Us to define together YOUR TECHNICAL SPECIFICATIONS.
Genetic tests
Mutation detection (SNP, deletion, insertion)
Ob Genotyping
- Technique: Real-time PCR
- The Ob mouse is a model for studying obesity and diabetes.
Only mice homozygous for the Lep point mutationOb are obese and are phenotypically different at around 4 weeks of age. Lean mice may be heterozygous (LepOb/Sara+) or wild (Lep)+/Sara+). - Scanelis has developed and validated a Lepob mutation genotyping assay for distinguishing Lep animalsOb/Sara+ and Lep+/Sara+.
It is also possible to use this test for early selection of Lep animalsOb/SaraOb. - Routine Analyses from Mouse Tail Fragments
- Lead time: 5 working days for 500 samples on schedule
- Pricing: Contact Us
Custom test(s)
- Technique: PCR/Real-time PCR
- Scanelis develops for you tailor-made tests for the detection of a particular mutation, according to your technical specifications.
- Scanelis validates the method from reference samples
- Scanelis carries out your routine analyses or transfers the method to you if you wish to treat it in-house
- Pricing: Contact Us
Determination of Integrated Transgene Copy Number in Transgenic Animals
- Technique: quantitative PCR (Real-time PCR)
- Scanelis develops and validates a test for detecting and quantifying your transgene by real-time PCR.
- Scanelis develops and validates a test for the detection and quantification of a reference gene in the animal species concerned. NB: some PCR tests for the detection and quantification of reference genes are already available from Scanelis (Contact Us)
- Scanelis validates the method for determining the copy number of the transgene under consideration from reference samples.
- The number of replicates of each PCR is determined with the customer to achieve the objectives set in terms of accuracy
- Scanelis carries out your routine analyses or transfers the method to you if you wish to treat it in-house
- Pricing: Contact Us
Example of a test developed by Scanelis: determination of the number of transgenes integrated in the transgenic rabbit. Download the poster presented at the FELASA 2007 congress: “ Accurate method for determination of transgene copy number using Real-time PCR in rabbits”.
Zygosity
- Technique: quantitative PCR (Real-time PCR)
- Differentiation of homozygous and heterozygous animals for a given transgene
- Scanelis develops and validates a test for detecting and quantifying your transgene by real-time PCR.
- Scanelis develops and validates a test for the detection and quantification of a reference gene in the animal species concerned. NB: some PCR tests for the detection and quantification of reference genes are already available from Scanelis (contact us)
- Scanelis validates the method for determining zygosity from reference samples
- The number of replicates of each PCR is determined with the customer to achieve the objectives set in terms of accuracy
- Scanelis carries out your routine analyses or transfers the method to you if you wish to treat it in-house
- Pricing: Contact Us
Expression Test
- Technique: quantitative PCR (Real-time PCR)
- Quantification of messenger RNAs of a gene or transgene
- Scanelis develops and validates a test for the detection and quantification of your transgene or the gene of interest, by real-time PCR.
- Scanelis validates the method
- Scanelis carries out your routine analyses or transfers the method to you if you wish to treat it in-house
- Pricing: Contact Us
Tests tailored to your needs
Custom PCR tests
Scanelis develops tailor-made tests to meet the needs of its customers (clinical trials, quality control for the pharmaceutical industry, etc.).
Stages of development
- the design & optimization are carried out in accordance with the customer's specific specifications
- the validation du test est ensuite réalisée par notre équipe R&D, qui vérifie la spécificité analytique du test sur des souches/échantillons de référence. La spécificité est également vérifiée par séquençage.
- The test is finally assessed according to the recommendations of the European Pharmacopoeia :
- the detection threshold which allows the sensitivity of the test to be assessed is determined by statistical studies (Probit analyses) in accordance with the recommendations of the European Pharmacopoeia. A detection limit of 95% is thus calculated. Suitable positive controls are used on each test run, allowing this detection limit to be verified for each analysis.
- the quantification threshold is determined,
- the reproducibility of the test is verified,
- the robustness of the test is validated.
Evaluation of a particular test
Scanelis can evaluate the performance of a particular test developed by its customer (detection limit, specificity, reproducibility, etc.).
Real-time PCR test catalog
All real-time PCR tests (real-time PCR) that we offer in the catalogue have been developed and validated by Scanelis' Research & Development team.
Our specifications are very strict:
- The test must be adapted to the needs of the field (quantitative aspect, typing of vaccine strains, detection of pathogenic strains).
- The analytical specificity of the test is evaluated on reference strains and field strains. It is verified by sequencing during the validation phases of the test.
- the detection threshold which allows the sensitivity of the test to be assessed is determined by statistical studies (Probit analyses) in accordance with the recommendations of the European Pharmacopoeia. A detection limit of 95% is thus calculated. Some appropriate positive controls are used on each test run, which can be used to verify this detection limit for each analysis. More details on the detection threshold
- the rate of asymptomatic carriers in the population as well as the level of carriage (viral or bacterial load) are also evaluated in the field in different populations in order to be able to assess the clinical interest of the test (diagnostic specificity), in particular compared to other veterinary diagnostic methods.
