Search for feline calicivirus by real-time RT-PCR. Very broad specificity of the test on feline caliciviruses (strains responsible for coryza, oral stomatitis, hypervirulent strains, etc.)
Test indications
- Evaluation of viral excretion and contagiousness of cats in the community.
- Determination of breeding status in breeding.
- Detection of asymptomatic carriers.
- Diagnostic de calicivirose in cats presenting clinical signs (coryza, oral stomatitis) and monitoring of viral excretion after clinical recovery.
Test characteristics
- Real-time RT-PCR
- Detection threshold
- Quantitative analysis viral load for determining the status of asymptomatic carrier animals
Samples to be taken
- Coryza : oropharyngeal / conjunctival / nasal cells taken with a cytobrush (especially if chronic disorders) or possibly a dry swab.
Other possible samples: chest effusion, lung (not fixed). - Hypervirulent systemic forms: they are accompanied by viremia, the EDTA blood sample is therefore appropriate, skin scraping if skin lesions.
- Oral stomatitis: oropharyngeal cells with a cytobrush at the level of the lesions.
- Determination of status of asymptomatic animals and follow-up after recovery: oropharyngeal cells (take from the tonsils)
Interpretation of the result
- Negative result : absence of the virus or quantity below the detection threshold of the technique. Allows the exclusion of calicivirus in a symptomatic animal.
- Positive result : presence of the virus in the sample. If the quantity of viral RNA is very low, we indicate this to you on the analysis report. Positive results must be interpreted in the clinical context and depending on the duration of development. It is necessary to take into account a asymptomatic carriage rate of 8% (with the test developed by Scanelis, in oral samples). Asymptomatic carriage may be more common in animals housed in communities (shelters in particular).
Do not hesitate to Contact Us for an interpretation of the quantitative result